Cambridge Protein Arrays

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CPA R&D Activities

Our Research Background at The Babraham Institute

 

Before setting up Cambridge Protein Arrays in 2010, we developed the  "DNA array to protein array" (DAPA) technology, which creates protein arrays by direct 'printing' from DNA arrays using cell-free protein expression systems.  (He M, Stoevesandt O, Palmer EA, Khan F, Ericsson O, Taussig MJ. Printing protein arrays from DNA arraysNature Methods. 2008 5:175-7.)

 

The basis of this method is that a template DNA array on one glass slide is sandwiched with a second, protein-capture slide, and cell-free protein synthesis is performed in a narrow gap between the two surfaces. Individual proteins synthesised in parallel from the arrayed DNA become immobilised through interaction with the protein-capturing reagent on the opposite surface. The result is a protein array replica of the DNA array, with good reproducibility and spot quality, as shown. DAPA makes possible the generation of protein arrays as and when required, in a single in situ reaction. By linking gene sequence data to protein expression, DAPA is an enabling technology for individualised or personalised proteomics. Similar methods were developed and taken forward to a sub-proteome scale by others (e.g. Ramachandran N, et al. Self-assembling protein microarrays. Science 2004; 305:86-90).