Proteome-Wide Enzyme Assays

 

HuProt™ application: Enzyme activity screening

HuProt™ human proteome microarrays are a powerful tool for profiling enzyme substrate specificity against the world’s largest arrayed collection of human proteins.  Protein-modifying enzymes are prime candidates for screening.

 

Assay setup

Array readout is by fluorescence, so the result of enzyme activity needs to be converted to a fluorescent signal. Assay options are as diverse as enzymes – please contact us to discuss the options and develop a strategy for your project.

 

References

 

Kinase assays:

 

• Hu J et al. (2015)  Systematic prediction of scaffold proteins reveals new design principles in scaffold-mediated signal transduction. PLoS Comput Biol 11(9):e1004508
• Tarrant MK et al. (2012). Regulation of CK2 by phosphorylation and O-GlcNAcylation revealed by semisynthesis.Nat Chem Biol 8(3):262-9.

 

Other enzyme assays:

 

• Xu Z et al. (2017) Systematic identification of the protein substrates of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T1/T2/T3 using a human proteome microarray.  Proteomics 17:11

• Cox E et al. (2015) Identification of SUMO E3 ligase-specific substrates using the HuProt human proteome microarray. Methods Mol Biol. 1295:455-63
• Lee YI et al. (2013). Protein microarray characterization of the S-nitrosoproteome. Mol Cell Proteomics 13:63-72.