Research and development of protein arrays and their applications in proteomics
Autoantibodies, leading to immune reactivity against specific tissues, are the cause of autoimmune diseases.
Identifying which antigens are the target of autoantibodies can be the first step to understanding the underlying disease mechanisms, as well as to disease stratification. Because of their uniquely broad content of human proteins, HuProt™ arrays present a strategy for uncovering the identity of as yet unknown autoantigens, or for the initial characterisation of conditions of suspected autoimmune aetiology.
Autoantibodies have also gained prominence as early diagnostic biomarkers in cancer, where they may emerge as an effect, rather than as the cause of the disease.
HuProt™ arrays provide the option to discover and identify signature autoantibodies through interaction with their arrayed antigens.
See here for literature references how HuProt™ arrays have been used !
Typically, diluted serum is incubated on the HuProt array, followed by secondary detection with a fluorophore-labelled anti-human Ig reagent.