Antibody Specificity Profiling
Research and development of protein arrays and their applications in proteomics
Confirm specificity, identify crossreactions.
Crossreactivity is an often overlooked issue with antibodies, with typical QC involving proof of binding to the intended target, but neglecting broad screens for potential crossreactivity. Screening your antibody of HuProt™ v3.0 arrays allows to identify potential crossreactivities across the 70% of the human proteome. This is of particularly high value for the characterisation of therapeutic or diagnostic candidate antibodies, where crossreactivities provide an early indication of possible off-target effects in vivo, or of potentially misleading diagnostic readouts. By eliminating candidate antibodies which show crossreactivities from the development process, significant savings in cost and time can be achieved.
Analogous to classical antibodies, alternative binder types can also be assayed on HuProt arrays.
Typically, the antibody of interest is incubated on the HuProt array, followed by a suitable fluorophore-labelled secondary reagent. Alternatively, if the antibody of interest is fluorophore-conjugated itself, incubation and detection can proceed in one step.