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Research and development of protein arrays and their applications in proteomics

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Antibody Specificity Profiling

 

HuProt™ application: Comprehensive antibody reactivity profiling

Confirm specificity, identify cross-reactions


Cross-reactivity is an often overlooked issue with antibodies, with typical QC involving proof of binding to the intended target, but neglecting broad screens for potential cross-reactivity. 

 

Screening your antibody of HuProt™ v4 arrays enables identification of potential cross-reactivities across the 80% of the human proteome. This is of particularly high value for the characterisation of therapeutic or diagnostic candidate antibodies, where cross-reactivities provide an early indication of possible off-target effects in vivo, or of potentially misleading diagnostic readouts. By eliminating candidate antibodies which show cross-reactivities from the development process, significant savings in cost and time can be achieved.


Analogous to classical antibodies, alternative binder types can also be assayed on HuProt™arrays.

 

See here for references of HuProt™ applications in binder profiling!

 

Assay workflow

 

Typically, the antibody of interest is incubated on the HuProt™ array, followed by a suitable fluorophore-labelled secondary reagent.  Alternatively, if the antibody of interest is fluorophore-conjugated itself, incubation and detection can proceed in one step.  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

GET IN TOUCH

 

Contact us to discuss the requirements for your particular experiment.

 

Special Offer: Order by 18th October to save 10% on Sales and Service

 

+44 (0)1223 496557

info@cambridgeproteinarrays.com